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inverted phase contrast microscope  (Olympus)


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    Structured Review

    Olympus inverted phase contrast microscope
    Inverted Phase Contrast Microscope, supplied by Olympus, used in various techniques. Bioz Stars score: 99/100, based on 3773 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/phase-contrast+microscope/CKX53+Cell+Culture+Microscope/pm41933720-52-8-13
    Average 99 stars, based on 3773 article reviews
    inverted phase contrast microscope - by Bioz Stars, 2026-09
    99/100 stars

    Images

    Related Articles

    Microscopy:

    Article Title: Establishment and characterization of a human pre-osteocyte cell line: hOsteo4-E9
    Article Snippet: Morphological changes in cells under FSS stimulation were analyzed using ImageJ software ( https://imagej.net/ij/ ). .. Phase contrast images were acquired with a culture microscope (CKX53, Olympus, Tokyo, Japan), and immunofluorescence (IF) images were captured with a confocal laser scanning microscope (FV3000, Olympus, Tokyo, Japan). ..

    Article Title: Sperm attraction by female reproductive fluid in a fish with an unconventional fertilisation strategy
    Article Snippet: After 50 seconds from sperm loading, 3μl liquid were collected from wells A and B and transferred to Eppendorf tubes. .. Sperm cells were then counted from each sample on a Makler chamber under an Olympus BX53 phase contrast microscope. ..

    Article Title: Broad-spectrum coronavirus inhibition by RSV fusion inhibitors targeting six-helix bundle formation.
    Article Snippet: Waning vaccine-derived immunity and the emergence of new SARS-CoV-2 subvariants continue to challenge global public health, emphasizing the need for novel inhibitors against SARS-CoV-2 infection.. Molecular inhibitors targeting virus entry are promising candidates as they can disrupt viral replication at the earliest stage.. In this study, we utilized a fluorescence-based SARS-CoV-2 pseudovirus screening platform to identify entry inhibitors from a repurposed library of bioactive compounds.

    Article Title: Inhibition of ZBTB7B-mediated ADPGK transcription by NEDD4 impedes glycolysis and progression of lung adenocarcinoma
    Article Snippet: .. Wound area images were captured at specified intervals using an Olympus CKX53 phase-contrast microscope. .. Plasmids and siRNAs were transfected following the manufacturer’s instructions using MegaTran 2.0 plasmid DNA transfection reagent (Origene, #TT210003) or siTran 2.0 siRNA transfection reagent (Origene, # TT210001).

    Article Title: Regulation of natural killer cell infiltration and tumor progression by CMTM6 in gastric cancer.
    Article Snippet: .. Images were captured using an inverted phase contrast microscope (CKX53, Olympus, Tokyo, Japan). .. For co-localization analysis, ImageJ (v2.16.0, National Institute of Health, USA) was used to obtain the pattern of fluorescence intensity under respective color channels.

    Article Title: Inhibition of ZBTB7B-mediated ADPGK transcription by NEDD4 impedes glycolysis and progression of lung adenocarcinoma.
    Article Snippet: .. Wound area images were captured at specified intervals using an Olympus CKX53 phase-contrast microscope. .. Plasmids and siRNAs were transfected following the manufacturer’s instructions using MegaTran 2.0 plasmid DNA transfection reagent (Origene, #TT210003) or siTran 2.0 siRNA transfection reagent (Origene, # TT210001).

    Article Title: Weathered plastic particles negatively affect mouse primary neurons and glial cells.
    Article Snippet: Extended author information available on the last page of the article Abstract The global increase in plastics production has raised significant concerns regarding plastic waste in marine and terrestrial ecosystems and potential human health risks.. Environmental weathering processes such as physical abrasion and ultraviolet (UV) irradiation cause plastic waste to fragment into tiny particles termed weathered nanoand microplastics (W-NMPs).. Despite their potential human health hazards, W-NMPs are underinvestigated in terms of their effects, particularly on the central nervous system.

    Article Title: Diatom microalgae-based living biophotovoltaics for high-performance photocurrent and hydrogen generation: indirect marine mucilage valorization approach.
    Article Snippet: • Marine mucilage–derived diatom microalgae served as living photoactive

    Immunofluorescence:

    Article Title: Establishment and characterization of a human pre-osteocyte cell line: hOsteo4-E9
    Article Snippet: Morphological changes in cells under FSS stimulation were analyzed using ImageJ software ( https://imagej.net/ij/ ). .. Phase contrast images were acquired with a culture microscope (CKX53, Olympus, Tokyo, Japan), and immunofluorescence (IF) images were captured with a confocal laser scanning microscope (FV3000, Olympus, Tokyo, Japan). ..

    Laser-Scanning Microscopy:

    Article Title: Establishment and characterization of a human pre-osteocyte cell line: hOsteo4-E9
    Article Snippet: Morphological changes in cells under FSS stimulation were analyzed using ImageJ software ( https://imagej.net/ij/ ). .. Phase contrast images were acquired with a culture microscope (CKX53, Olympus, Tokyo, Japan), and immunofluorescence (IF) images were captured with a confocal laser scanning microscope (FV3000, Olympus, Tokyo, Japan). ..

    Infection:

    Article Title: Broad-spectrum coronavirus inhibition by RSV fusion inhibitors targeting six-helix bundle formation.
    Article Snippet: Waning vaccine-derived immunity and the emergence of new SARS-CoV-2 subvariants continue to challenge global public health, emphasizing the need for novel inhibitors against SARS-CoV-2 infection.. Molecular inhibitors targeting virus entry are promising candidates as they can disrupt viral replication at the earliest stage.. In this study, we utilized a fluorescence-based SARS-CoV-2 pseudovirus screening platform to identify entry inhibitors from a repurposed library of bioactive compounds.

    Isolation:

    Article Title: Weathered plastic particles negatively affect mouse primary neurons and glial cells.
    Article Snippet: Extended author information available on the last page of the article Abstract The global increase in plastics production has raised significant concerns regarding plastic waste in marine and terrestrial ecosystems and potential human health risks.. Environmental weathering processes such as physical abrasion and ultraviolet (UV) irradiation cause plastic waste to fragment into tiny particles termed weathered nanoand microplastics (W-NMPs).. Despite their potential human health hazards, W-NMPs are underinvestigated in terms of their effects, particularly on the central nervous system.



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    Alizarin Red S staining and ARS quantification of mineralized matrix in DPSCs cultured under different conditions for 7 and 14 days. a Representative images show calcium deposition in DPSCs cultured in osteogenic differentiation medium (ODM) alone (control), ODM supplemented with a low dose of Metformin (500 µM), or a high dose of Metformin (1000 µM). The magnification is 20×, and the scale bar is 50 μm. Images were captured using the LABOMED Trinocular inverted phase contrast microscope model <t>TCM400</t> and Atlas 16MP CMOS USB Camera with PixelPro 3.0 software (LABOMED, USA). b Bar graphs show ARS concentrations representing mineral deposition at days 7 and 14. Error bars represent standard deviations. Statistical significance was assessed using one-way ANOVA followed by Tukey’s post-hoc test (a: vs. ODM; b: vs. low dose of Metformin; c: vs. high dose of Metformin. Abbreviations: DPSCs: Dental pulp-derived mesenchymal stem cells, ODM: Osteogenic differentiation medium, MET: Metformin, ARS: Alizarin red stain
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    Alizarin Red S staining and ARS quantification of mineralized matrix in DPSCs cultured under different conditions for 7 and 14 days. a Representative images show calcium deposition in DPSCs cultured in osteogenic differentiation medium (ODM) alone (control), ODM supplemented with a low dose of Metformin (500 µM), or a high dose of Metformin (1000 µM). The magnification is 20×, and the scale bar is 50 μm. Images were captured using the LABOMED Trinocular inverted phase contrast microscope model <t>TCM400</t> and Atlas 16MP CMOS USB Camera with PixelPro 3.0 software (LABOMED, USA). b Bar graphs show ARS concentrations representing mineral deposition at days 7 and 14. Error bars represent standard deviations. Statistical significance was assessed using one-way ANOVA followed by Tukey’s post-hoc test (a: vs. ODM; b: vs. low dose of Metformin; c: vs. high dose of Metformin. Abbreviations: DPSCs: Dental pulp-derived mesenchymal stem cells, ODM: Osteogenic differentiation medium, MET: Metformin, ARS: Alizarin red stain
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    Alizarin Red S staining and ARS quantification of mineralized matrix in DPSCs cultured under different conditions for 7 and 14 days. a Representative images show calcium deposition in DPSCs cultured in osteogenic differentiation medium (ODM) alone (control), ODM supplemented with a low dose of Metformin (500 µM), or a high dose of Metformin (1000 µM). The magnification is 20×, and the scale bar is 50 μm. Images were captured using the LABOMED Trinocular inverted phase contrast microscope model <t>TCM400</t> and Atlas 16MP CMOS USB Camera with PixelPro 3.0 software (LABOMED, USA). b Bar graphs show ARS concentrations representing mineral deposition at days 7 and 14. Error bars represent standard deviations. Statistical significance was assessed using one-way ANOVA followed by Tukey’s post-hoc test (a: vs. ODM; b: vs. low dose of Metformin; c: vs. high dose of Metformin. Abbreviations: DPSCs: Dental pulp-derived mesenchymal stem cells, ODM: Osteogenic differentiation medium, MET: Metformin, ARS: Alizarin red stain
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    Alizarin Red S staining and ARS quantification of mineralized matrix in DPSCs cultured under different conditions for 7 and 14 days. a Representative images show calcium deposition in DPSCs cultured in osteogenic differentiation medium (ODM) alone (control), ODM supplemented with a low dose of Metformin (500 µM), or a high dose of Metformin (1000 µM). The magnification is 20×, and the scale bar is 50 μm. Images were captured using the LABOMED Trinocular inverted phase contrast microscope model <t>TCM400</t> and Atlas 16MP CMOS USB Camera with PixelPro 3.0 software (LABOMED, USA). b Bar graphs show ARS concentrations representing mineral deposition at days 7 and 14. Error bars represent standard deviations. Statistical significance was assessed using one-way ANOVA followed by Tukey’s post-hoc test (a: vs. ODM; b: vs. low dose of Metformin; c: vs. high dose of Metformin. Abbreviations: DPSCs: Dental pulp-derived mesenchymal stem cells, ODM: Osteogenic differentiation medium, MET: Metformin, ARS: Alizarin red stain
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    Alizarin Red S staining and ARS quantification of mineralized matrix in DPSCs cultured under different conditions for 7 and 14 days. a Representative images show calcium deposition in DPSCs cultured in osteogenic differentiation medium (ODM) alone (control), ODM supplemented with a low dose of Metformin (500 µM), or a high dose of Metformin (1000 µM). The magnification is 20×, and the scale bar is 50 μm. Images were captured using the LABOMED Trinocular inverted phase contrast microscope model <t>TCM400</t> and Atlas 16MP CMOS USB Camera with PixelPro 3.0 software (LABOMED, USA). b Bar graphs show ARS concentrations representing mineral deposition at days 7 and 14. Error bars represent standard deviations. Statistical significance was assessed using one-way ANOVA followed by Tukey’s post-hoc test (a: vs. ODM; b: vs. low dose of Metformin; c: vs. high dose of Metformin. Abbreviations: DPSCs: Dental pulp-derived mesenchymal stem cells, ODM: Osteogenic differentiation medium, MET: Metformin, ARS: Alizarin red stain
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    Alizarin Red S staining and ARS quantification of mineralized matrix in DPSCs cultured under different conditions for 7 and 14 days. a Representative images show calcium deposition in DPSCs cultured in osteogenic differentiation medium (ODM) alone (control), ODM supplemented with a low dose of Metformin (500 µM), or a high dose of Metformin (1000 µM). The magnification is 20×, and the scale bar is 50 μm. Images were captured using the LABOMED Trinocular inverted phase contrast microscope model <t>TCM400</t> and Atlas 16MP CMOS USB Camera with PixelPro 3.0 software (LABOMED, USA). b Bar graphs show ARS concentrations representing mineral deposition at days 7 and 14. Error bars represent standard deviations. Statistical significance was assessed using one-way ANOVA followed by Tukey’s post-hoc test (a: vs. ODM; b: vs. low dose of Metformin; c: vs. high dose of Metformin. Abbreviations: DPSCs: Dental pulp-derived mesenchymal stem cells, ODM: Osteogenic differentiation medium, MET: Metformin, ARS: Alizarin red stain
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    Image Search Results


    Alizarin Red S staining and ARS quantification of mineralized matrix in DPSCs cultured under different conditions for 7 and 14 days. a Representative images show calcium deposition in DPSCs cultured in osteogenic differentiation medium (ODM) alone (control), ODM supplemented with a low dose of Metformin (500 µM), or a high dose of Metformin (1000 µM). The magnification is 20×, and the scale bar is 50 μm. Images were captured using the LABOMED Trinocular inverted phase contrast microscope model TCM400 and Atlas 16MP CMOS USB Camera with PixelPro 3.0 software (LABOMED, USA). b Bar graphs show ARS concentrations representing mineral deposition at days 7 and 14. Error bars represent standard deviations. Statistical significance was assessed using one-way ANOVA followed by Tukey’s post-hoc test (a: vs. ODM; b: vs. low dose of Metformin; c: vs. high dose of Metformin. Abbreviations: DPSCs: Dental pulp-derived mesenchymal stem cells, ODM: Osteogenic differentiation medium, MET: Metformin, ARS: Alizarin red stain

    Journal: BMC Oral Health

    Article Title: Dose-dependent effects of Metformin on proliferation and odontogenic differentiation of dental pulp stem cells

    doi: 10.1186/s12903-026-08221-w

    Figure Lengend Snippet: Alizarin Red S staining and ARS quantification of mineralized matrix in DPSCs cultured under different conditions for 7 and 14 days. a Representative images show calcium deposition in DPSCs cultured in osteogenic differentiation medium (ODM) alone (control), ODM supplemented with a low dose of Metformin (500 µM), or a high dose of Metformin (1000 µM). The magnification is 20×, and the scale bar is 50 μm. Images were captured using the LABOMED Trinocular inverted phase contrast microscope model TCM400 and Atlas 16MP CMOS USB Camera with PixelPro 3.0 software (LABOMED, USA). b Bar graphs show ARS concentrations representing mineral deposition at days 7 and 14. Error bars represent standard deviations. Statistical significance was assessed using one-way ANOVA followed by Tukey’s post-hoc test (a: vs. ODM; b: vs. low dose of Metformin; c: vs. high dose of Metformin. Abbreviations: DPSCs: Dental pulp-derived mesenchymal stem cells, ODM: Osteogenic differentiation medium, MET: Metformin, ARS: Alizarin red stain

    Article Snippet: Images were captured using the LABOMED Trinocular inverted phase contrast microscope model TCM400 and Atlas 16MP CMOS USB Camera with PixelPro 3.0 software (LABOMED, USA). b Bar graphs show ARS concentrations representing mineral deposition at days 7 and 14.

    Techniques: Staining, Cell Culture, Control, Microscopy, Software, Derivative Assay